Class 2 Family 6

Family 6 Overview

2 experimentally studied proteins

2 sequences in Swiss-Prot

1,905 unique sequences in UniRef100

Bacterial isoaspartyl peptidases

Clan 2: Family 6

Reference Monomer¹

Reference Structure

Family 6 Motifs
(P74383)

Experimentally Studied Proteins

	Fam ? Class - Clan - Family	Alt ? Alternative historical name / classification	AN ? UniProt accession number	Name ? UniProt entry name, only given here for Swiss-Prot entries	EC	Organism	Cell-Loc	AAs	Structure	PDB	K_m i for Asn [mM]	V_max i for Asn [μmol/min/mg]	K_cat i for Asn [s^-1]
	2-2-6	-	Q8YQB1	ASGX_NOSS1	3.4.19.5	Nostoc sp.	-	318	-	-	2.0	2.2	-
	2-2-6	-	P74383	ASGX_SYNY3	3.4.19.5	Synechocystis sp.	-	329	-	-	0.66	1.6	-

Swiss-Prot Sequences

	Fam ? Class - Clan - Family	Alt ? Alternative historical name / classification	AN ? UniProt accession number	Name ? UniProt entry name, only given here for Swiss-Prot entries	EC	Organism	Cell-Loc	AAs	Structure	PDB	K_m i for Asn [mM]	V_max i for Asn [μmol/min/mg]	K_cat i for Asn [s^-1]
	2-2-6	-	Q8YQB1	ASGX_NOSS1	3.4.19.5	Nostoc sp.	-	318	-	-	2.0	2.2	-
	2-2-6	-	P74383	ASGX_SYNY3	3.4.19.5	Synechocystis sp.	-	329	-	-	0.66	1.6	-

UniRef100 Sequences²

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Representative Sequence Alignment

Additional Information

Clan 2 only contains one family: Family 6. Although it is not immediately obvious from the phylogenetic tree, sequences in this family are structurally and phylogenetically distant from neighbouring families and even from families that catalyse the same reaction. Family 6 enzymes are bacterial isoaspartyl peptidases, enzymes that hydrolyse a range of isoaspartyl dipeptides and l‑asparagine. Interestingly, the Synechocystis sp. isoaspartyl peptidase (P74383) technically has a Km for l‑asparagine in the higher micromolar range, however isoaspartyl dipeptides are nonetheless the preferred substrates.

¹Varadi, M et al. AlphaFold Protein Structure Database in 2024: providing structure coverage for over 214 million protein sequences. Nucleic Acids Research (2024). Licensed under CC BY 4.0.

²Suzek, B.E. et al. UniRef: comprehensive and non-redundant UniProt reference clusters. Bioinformatics (2007). Licensed under CC BY 4.0. Added classification code to sequence headers.