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Class 2 Family 2

Family 2 Overview

4 experimentally studied proteins

23 sequences in Swiss-Prot

3,107 unique sequences in UniRef100

Eukaryotic aspartylglucosaminidases

Clan 1: Family 1, Family 2, Family 3, Family 4, Family 5

Reference Monomer1

mono2-2.png

Reference Structure

1APY.png

Family 2 Motifs
(P20933)

motifs2-2

Experimentally Studied Proteins

Fam ? Class - Clan - Family Alt ? Alternative historical name / classification AN ? UniProt accession number Name ? UniProt entry name, only given here for Swiss-Prot entries EC Organism Cell-Loc AAs Structure PDB Km i for Asn [mM] Vmax i for Asn [μmol/min/mg] Kcat i for Asn [s-1]
2-1-2 - D0V0N4 3.5.1.26 Asobara tabida Venom 362 - - 4.051 - -
2-1-2 - P20933 ASPG_HUMAN 3.5.1.26 Homo sapiens Lysosome 346 Hetero tetramer / homo dimer 1APY PDBs 0.656 i Sequence may not be exact match - -
2-1-2 - A0A141NXG8 3.5.1.26 Leptopilina heterotoma Venom 367 - - 1.188 - -
2-1-2 - O02467 ASPG_SPOFR 3.5.1.26 Spodoptera frugiperda Lysosome 320 - - 3.0 - -

Swiss-Prot Sequences

Fam ? Class - Clan - Family Alt ? Alternative historical name / classification AN ? UniProt accession number Name ? UniProt entry name, only given here for Swiss-Prot entries EC Organism Cell-Loc AAs Structure PDB Km i for Asn [mM] Vmax i for Asn [μmol/min/mg] Kcat i for Asn [s-1]
2-1-2 - Q56W64 ASPG3_ARATH 3.4.19.5 Arabidopsis thaliana - 359 - - - - -
2-1-2 - Q21697 ASPG_CAEEL 3.5.1.26 Caenorhabditis elegans Lysosome 363 - - - - -
2-1-2 - B3MJ16 ASPG2_DROAN 3.5.1.26 Drosophila ananassae - 378 - - - - -
2-1-2 - B3N6Y7 ASPG1_DROER 3.5.1.26 Drosophila erecta - 396 - - - - -
2-1-2 - B3NN96 ASPG2_DROER 3.5.1.26 Drosophila erecta - 399 - - - - -
2-1-2 - B4JVW6 ASPG1_DROGR 3.5.1.26 Drosophila grimshawi - 393 - - - - -
2-1-2 - Q8MR45 ASPG1_DROME 3.5.1.26 Drosophila melanogaster - 393 - - - - -
2-1-2 - Q9W2C3 ASPG2_DROME 3.5.1.26 Drosophila melanogaster - 397 - - - - -
2-1-2 - B4GGF2 ASPG1_DROPE 3.5.1.26 Drosophila persimilis - 388 - - - - -
2-1-2 - B4GHE3 ASPG2_DROPE 3.5.1.26 Drosophila persimilis - 457 - - - - -
2-1-2 - Q28Y14 ASPG1_DROPS 3.5.1.26 Drosophila pseudoobscura - 388 - - - - -
2-1-2 - Q28XQ5 ASPG2_DROPS 3.5.1.26 Drosophila pseudoobscura - 457 - - - - -
2-1-2 - B4HT15 ASPG1_DROSE 3.5.1.26 Drosophila sechellia - 393 - - - - -
2-1-2 - B4I7X1 ASPG2_DROSE 3.5.1.26 Drosophila sechellia - 397 - - - - -
2-1-2 - B4QHB1 ASPG1_DROSI 3.5.1.26 Drosophila simulans - 393 - - - - -
2-1-2 - B4QGM0 ASPG2_DROSI 3.5.1.26 Drosophila simulans - 397 - - - - -
2-1-2 - B4NWI1 ASPG1_DROYA 3.5.1.26 Drosophila yakuba - 396 - - - - -
2-1-2 - B4P8E0 ASPG2_DROYA 3.5.1.26 Drosophila yakuba - 399 - - - - -
2-1-2 - P20933 ASPG_HUMAN 3.5.1.26 Homo sapiens Lysosome 346 Hetero tetramer / homo dimer 1APY PDBs 0.656 i Sequence may not be exact match - -
2-1-2 - Q4R6C4 ASPG_MACFA 3.5.1.26 Macaca fascicularis Lysosome 346 - - - - -
2-1-2 - Q64191 ASPG_MOUSE 3.5.1.26 Mus musculus Lysosome 346 - - - - -
2-1-2 - P30919 ASPG_RAT 3.5.1.26 Rattus norvegicus Lysosome 345 - - - - -
2-1-2 - O02467 ASPG_SPOFR 3.5.1.26 Spodoptera frugiperda Lysosome 320 - - 3.0 - -

UniRef100 Sequences2

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Representative Sequence Alignment

Motifs Image

Additional Information

Clan 1 contains five families that are to an extent phylogenetically distinct from the other clans, they have aspartylglucosaminidase (AGA) activity in addition to l‑asparaginase activity.

Family 2 is well-studied and contains sequences of eukaryotic origin. One protein structure has been solved, the lysosomal Homo sapiens AGA (P20933), its Km for l‑asparagine is about four times higher than for aspartylglucosamines.

1Varadi, M et al. AlphaFold Protein Structure Database in 2024: providing structure coverage for over 214 million protein sequences. Nucleic Acids Research (2024). Licensed under CC BY 4.0.

2Suzek, B.E. et al. UniRef: comprehensive and non-redundant UniProt reference clusters. Bioinformatics (2007). Licensed under CC BY 4.0. Added classification code to sequence headers.


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